Figure 3.

Thioalbamide inhibits cell cycle progression. (a) Cytofluorimetric analysis of MCF7 cells treated for 72 h with DMSO (control) or 50 nM thioalbamide and stained with PI; graphs represent the percentage of total events in the samples (highlighting the sub-G1 peak). (b) Cell cycle distribution of MCF7 cells (excluding sub-G1 events). (c) Immunoblot analysis of the main regulators of G1/S phase transition and quantification of their expression levels by densitometry. Note that thioalbamide treatment induces cell cycle arrest at the G1/S checkpoint. Values represent mean ± SD of three independent experiments. **** P value < 0.0001.