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. 2019 Nov 6;8(11):1887. doi: 10.3390/jcm8111887

Figure 2.

Figure 2

Vglut2 mRNA and Tyrosine hydroxylase (TH) immunoreactivity in midbrain DA neurons. (A–D) In-situ hybridization for Vglut2 (VG2) mRNA (black) combined with fluorescent immunohistochemistry for TH (green) on sagittal sections of an E12.5 embryo. Vglut2 mRNA is detected in multiple regions in the embryo, including the ventral midbrain (MB) where DA neurons develop (A,B). Vglut2 mRNA is co-localized with TH-immunoreactivity in DA neurons within the MB (C,D). Arrows indicate Vglut2-positive cytoplasm (B) and Vglut2/TH double-positive neurons (D) (Magnification: B,D 300x). Reprinted from Birgner et al., 2010 [54].