Figure 4.

Effect of AgNP exposure and ionizing radiation (IR) on mitochondrial ROS. (a) Mitochondrial H₂O₂. The cells were treated with 10 μg/mL AgNPs for 24 h and 2 Gy IR immediately after the start of AgNP exposure, stained with MitoPY1 and analyzed by flow cytometry. (b) Mitochondrial superoxide. The cells were treated with 10 μg/mL AgNPs for 24 h and 2 Gy IR immediately after the start of the AgNP exposure, stained with MitoSOX, and analyzed by flow cytometry. Data are presented as mean fluorescence value, the error bars represent the standard error of the mean. * p = 0.01–0.05, ** p = 0.001–0.01, *** p < 0.001, calculated relative to the untreated conditions using Students’ t-test.