Figure 5.
DMF induces apoptosis and caspase-3/7 activation in T84 but not HT-29 cells. Analysis of apoptosis using the Cell Death Detection Kit. (a) HT-29 cells were treated for 24 h with DMF at the indicated concentrations; (b) T84 cells were treated for 24 h with DMF at the indicated concentrations. Analysis of caspase-3/7 activity; (c) HT-29 cells were treated for 24 h with DMF at the indicated concentrations; and (d) T84 cells were treated for 24 h with DMF at the indicated concentrations. Mean values from at least three independent experiments are shown as mean ± SD. * p < 0.05: significant. As a positive control, staurosporine (SSP) 1 µM was used. DMSO 0.2% served as a solvent control.