Table 1.
Primers for deletion plasmids construction from the Hu sheep BMP7 promoter.
| Name | Sequence (5′–3′) | Product Length/bp |
|---|---|---|
| B1 | F: GGGGTACCCCAAGTGGGCAACTCAGTGTC | 1757 |
| R: CCAAGCTTGTACAGGTCCAGCATGAACATGG | ||
| B2 | F: GGGGTACCCAGTACAAAGGCAAACTGGCAACA | 1014 |
| B3 | F: GGGGTACCCGCTCGTATTCCCCTCTCCGCATC | 787 |
| B4 | F: GGGGTACCCCCAGGCCCCAGCGCGTACCA | 574 |
| R: CCAAGCTTCCAGAGCGCCACGAAGCTGT | ||
| B5 | F: GGGGTACCCGAGTCCGGAGAAGGCAGG | 214 |
| R: CCAAGCTTGCTGGGGCCTGGGAGGAGGA |
Note that the underline represents enzyme sites. B2, B3, and B4 have the same reverse primer.