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Illustration of the main strategies used to differentiate hiPSC into hiPSC-ECs. Embryoid Bodies (EBs): cultured in suspension hiPSCs tend to auto-aggregate in embryoid bodies. Co-culture: hiPSCs are co-cultured with cells able to guide their differentiation into the mature phenotype. Two-dimensional (2D) monolayer culture: hiPSCs are seeded on matrix-coated plates where they are induced to differentiate.
