Figure 5.
ASH promotes DNA damage and H2A.X phosphorylation in HepG2 cell. (A) DNA Damage caused by 1–2.5 µM ASH was measured by the Comet Assay for 20 min. Tail moment was used to measure the double strand breaks by calculating the percentage of tail DNA x tail length. * p < 0.05; ** p < 0.01. (B) HepG2 cells were pretreated with 1–10 µM ASH for 12 h, the expression of phospho-H2A.X-Ser139 and H2A.X were analyzed by Western blot assay. Control group received an equal amount of vehicle (0.01% DMSO). (C) HepG2 cells were pretreated with 2.5 µM ASH for 0, 1, 2, 4, 8, 16, and 24 h. The expressions of phospho-H2A.X-Ser139 and H2A.X were analyzed by Western blot assay. (D) HepG2 cells were pre-incubated with ASH at various dosages for 12 h. The expression of DR-4 and DR-5 was evaluated by Western blotting assay.