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Multiplex gene editing using CRISPR system Yersinia (Csy4) endonuclease, shown here as blue circles. Csy4 restriction sites are cloned between each sgRNA, and Csy4 endonuclease gene is also cloned in the same vector. Expression of Csy4 endonuclease results in the separation of individual sgRNAs, which can then go on to target their respective sites.
