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. 2019 Nov 11;8(11):543. doi: 10.3390/antiox8110543

Figure 2.

Figure 2

Image of a retina from a postnatal 11-day rd1 mouse, showing immunostaining for neuronal nitric oxide synthase (nNOS). Immunofluorescent staining was performed on retinal cryosections that were incubated overnight with the primary antibody anti-neuronal nitric oxide synthetase (anti-nNOS) (1:200, Santacruz Biotechnology, Dallas, USA). The sections were later incubated with the fluorescence-conjugated secondary antibody Alexa Fluor 488 (Invitrogen, Life Technologies, Madrid, Spain). (Scale: 200 μm).