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. 2019 Oct 27;8(11):1327. doi: 10.3390/cells8111327

Figure 1.

Figure 1

In vitro culture of matched normal and tumor conditionally reprogrammed cell cultures (CRCs). (A) Cell morphology: under light microscope the non-malignant cells appeared to grow in tight colonies (inner square) surrounded by feeder cells, a characteristic of cells cultured in CRC, whereas malignant cells grew as individual big cells (inner square) displaying prominent intercellular junctions. Non-malignant cells exhibited a heterogeneous population composed of small, dark, hexagonal cells and some big and flat, while tumor cells displayed a highly homogenous population of big, dark, hexagonal cells (outer squares). Bars: 50 and 200 µm. (B) Karyotype: cytogenetic analysis of normal and tumor CRCs were performed at early passage. The non-malignant line (46, XX) displayed a diploid karyotype with no apparent chromosomal alterations (left) whereas the malignant line consisted of a near-triploid clone karyotype (74, XXX, +1, +1, +1, +2, +2, +3, +3, +4, +5, +6, +6, +7, +7, +10, +10, +11, +11, +12, +16, +16, +17, +17, +18, +19, +19, +20, +21, +22) (right).