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. 2019 Oct 27;8(11):1327. doi: 10.3390/cells8111327

Figure 2.

Figure 2

Organoid cultures of normal and tumor CRCs in matrigel. Morphogenesis: normal CRC (A) formed small spheres of approximately 100 µm characterized by displaying only one lobule with a mass-like shape and central growth, while tumor CRC (C) formed more prominent spheres of more than 200 µm consisting of multiple lobules with a grape-like shape and defined edges. Bars 100 µm. Unlike normal CRC that grew as independent spheres (B), tumor CRC developed invasive processes (D) which communicated adjacent spheres. Bars 400 µm. Immunohistochemistry: spheres from on-malignant cells depicted a polarized central growth with a structure similar to an arrested acinus (E) whereas the spheres from malignant cells were formed by actively proliferating cells (I) correlated with Ki-67 where the non-malignant displayed a negative staining (G) but stained moderately positive to P63 (H) while a high percentage of malignant cells stained strongly positive for Ki-67 (K) and the remaining for P63 (L). All the spheres from both lines stained strongly and diffusely positive for CK (cytokeratin) 14. Bars: 100 µm.