Table 2.
Recombinant Canine Cytokines for ex vivo NK Expansion.
| Study | Year | Starting Population | Feeder Cells | Media | Additives | Human Cytokines | Canine Cytokine | Days | |
|---|---|---|---|---|---|---|---|---|---|
| Foltz | 2016 | CD3−/ NKp46+ cells from healthy dog PBMCs |
Irradiated K562 Clone9. mbIL-21 cells | 100 IU/mL rh-IL2 * | 6.1 ng/ mL rcIL-2 * |
Not used in combination with human cytokines | 21 days | ||
| Lee | 2015 | Healthy dog isolated PBMCs | 100-Gy-irradiated K562 cells | RPMI | fetal bovine serum, penicillin, streptomycin |
100 IU/mL rhIL-2 10 IU/ml rhIL-15 |
10 IU/mL rcIL-15 | Used in combination with rhIL-2 | 21 days |
| Shin | 2015 | Healthy dog isolated PBMCs | 100-Gy-irradiated K562 cells | RPMI 1640 | fetal bovine serum, penicillin, streptomycin |
100 IU/mL rhIL-2 10 IU/mL rhIL-15 |
5 ng/mL rcIL-21 | Used in combination with rhIL-2 and rhIL-15 | 21 days |
* rh = recombinant human, rc = recombinant canine; CD, PBMCs, RPMI,