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Cycle of the procedure of CAR-T cell production and infusion. Commencing with the harvesting of peripheral blood, from which T cells are separated by leukapheresis. The T lymphocytes are transfected by a viral or non-viral vector, e.g., retrovirus, carrying the genes encoding the CAR sequence in order to modify the T cells with the CAR component. The CAR-T cells undergo expansion and are then infused into the patient.
