. Author manuscript; available in PMC: 2019 Dec 16.

Published in final edited form as: Arch Biochem Biophys. 2001 Mar 15;387(2):243–249. doi: 10.1006/abbi.2000.2259

TABLE I.

Spectral Properties of Trp-26 in TnCF26W

	TnCF26W			TnCF26W-dCW-TnI			TnCF26W-TnI(1–159)
	EGTA	Mg²⁺	Ca²⁺	EGTA	Mg²⁺	Ca²⁺	EGTA	Mg²⁺	Ca²⁺
λ_max (nm)	343	342	345	344	344	330	344	344	330
Q	0.12	0.12	0.39	0.11	0.11	0.28	0.091	0.095	0.2l
τ_mean (ns)	3.25	3.74	7.08	3.24	3.52	5.03	3.30	3.51	5.0l
τ1 (ns)	3.34	5.14	7.26	3.47	6.10	5.22	3.42	4.14	5.16
τ2 (ns)	0.40	2.75	1.48	0.49	3.04	0.66	0.40	2.57	0.68
τ3 (ns)		0.31			0.34			0.2l
A1	0.80	0.55	0.86	0.63	0.07	0.75	0.73	0.40	0.80
A2	0.20	0.21	0.14	0.37	0.54	0.25	0.27	0.36	0.20
A3		0.24			0.38			0.24

Note. A_i, fractional amplitude of component lifetime τ_i; Q, quantum yield; λ_max, wavelength at which maximum fluorescence emission was observed; τ_mean, mean lifetime.