Table 1. Representative Assays and Techniques for Assessment LDD in Cells.
assay | cell lysates | live cells | target engagement | ternary complex formation | ubiquitination | proteosome trafficking | degradation |
---|---|---|---|---|---|---|---|
Western blot | √ | √ | |||||
enzyme-linked immunosorbent assay (ELISA) | √ | √ | |||||
multiplexed immunoassays | √ | √ | |||||
proteomics | √ | √ | √ | ||||
enzyme fragment complementation | √ | √ | √ | ||||
protein fusion tags | √ | √ | √ | ||||
bioluminescence | √ | √ | √ | √ | √ | √ | √ |