Fig. 2.

Northern blot analysis of mitochondrial tRNA. a Equal amounts of total mitochondrial RNA from various cell lines were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with DIG-labeled oligonucleotide probes specific for the tRNA^Thr, tRNA^His, tRNA^Glu, tRNA^Ala respectively. b Quantification of tRNA levels. Average relative tRNA content per cell, was normalized to the average content per cell of 5S rRNA in three mutant cybrid cell lines(III1–3, III1–5 and III1–8) carrying the 15910C > T and control cybrid cell lines (C2–2, C2–8 and C2–9). The values for the latter are expressed as percentages of the average values for the control cell lines