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. 2019 Nov 26;8(23):e013112. doi: 10.1161/JAHA.119.013112

Table 1.

Prime Sequence for RT‐qPCR

Gene Primer Sequence
miR‐9a‐5p F: 5′‐ACGGCGGTCTTTGGTTATCTA‐3′
R: 5′‐CAGTGCAGGGTCCGAGGTAT‐3′
miR‐18a‐5p F: 5′‐ACGTAAGGTGCATCTAGTGCAGATA‐3′
R: 5′‐GTGCAGGGTCCGAGGT‐3′
miR‐199a‐3p F: 5′‐GCCCCAGTGTTCAGACTACC‐3′
R: 5′‐CTGGCCCTTGCCCAGTCTAA‐3′
miR‐144a‐3p F: 5′‐TACTGCATCAGGAACTGACTGGA‐3′
R: 5′‐GTGCAGG GTCCGAGGT‐3′
miR‐128‐3p F: 5′‐CAGCCGTCACAGTGAACCG‐3′
R: 5′‐TCCACACCACCTGAGCCG‐3′
miR‐27b‐3p F: 5′‐TCTAGATGCTCCTCCAGAAACCGTGG‐3′
R: 5′‐GAATTCCCTTTCAGACAGATGTCCGA‐3′
miR‐27a‐3p F: 5′‐TTCACAGTGGCTAAGTTCCGC‐3′
R: 5′‐AGGGCTTAGCTGCTTGTGAGCA‐3′
miR‐101 F: 5′‐CTGTGCAGCAAACAGTCCAC‐3′
R: 5′‐AGAGAGGGTGCTACAGGAG‐3′
RUNX1 F: 5′‐CTCCGTGCTACCCACTCACT‐3′
R: 5′‐GTCGTTGAATCTCGCTACCTG‐3′
RANTES F: 5′‐CCCCATATGGCTCGGACACCA‐3′
R: 5′‐CTAGCTCATCTCCAATATGTTGAT‐3′
CCR5 F: 5′‐AACCTGGCCATCTCTGACCTG‐3′
R: 5′‐GTAGCAGATGACCATGAC‐3′
TGF‐β1 F: 5′‐TCGACATGGAGCTGGTGAAA‐3′
R: 5′‐GAGCCTTAGTTTGGACAGGATCTG‐3′
Smad2 R: 5′‐GTGTTTGCCGAGTGCCTAAGT‐3′
R: 5′‐TTACAGCCTGGTGGGATTTG‐3′
U6 F: 5′‐CTCGCTTCGGCAGCACA‐3′
R: 5′‐AACGCTTCACGAATTTGCGT‐3′
β‐actin F: 5′‐GGCATGGGTCAGAAGGATTCC‐3′
R: 5′‐ATGTCACGCACGATTTCCCGC‐3′

CCR5 indicates chemokine receptor 5; F, forward; miR, microRNA; R, reverse; RANTES, regulated upon activation normal T‐cell expressed and secreted; RT‐qPCR, reverse transcription quantitative polymerase chain reaction; RUNX1, runt‐related transcription factor 1; Smad2, Smad family member 2; TGF‐β1, transforming growth factor β1.