Table 1.
Gene | Primer Sequence |
---|---|
miR‐9a‐5p | F: 5′‐ACGGCGGTCTTTGGTTATCTA‐3′ |
R: 5′‐CAGTGCAGGGTCCGAGGTAT‐3′ | |
miR‐18a‐5p | F: 5′‐ACGTAAGGTGCATCTAGTGCAGATA‐3′ |
R: 5′‐GTGCAGGGTCCGAGGT‐3′ | |
miR‐199a‐3p | F: 5′‐GCCCCAGTGTTCAGACTACC‐3′ |
R: 5′‐CTGGCCCTTGCCCAGTCTAA‐3′ | |
miR‐144a‐3p | F: 5′‐TACTGCATCAGGAACTGACTGGA‐3′ |
R: 5′‐GTGCAGG GTCCGAGGT‐3′ | |
miR‐128‐3p | F: 5′‐CAGCCGTCACAGTGAACCG‐3′ |
R: 5′‐TCCACACCACCTGAGCCG‐3′ | |
miR‐27b‐3p | F: 5′‐TCTAGATGCTCCTCCAGAAACCGTGG‐3′ |
R: 5′‐GAATTCCCTTTCAGACAGATGTCCGA‐3′ | |
miR‐27a‐3p | F: 5′‐TTCACAGTGGCTAAGTTCCGC‐3′ |
R: 5′‐AGGGCTTAGCTGCTTGTGAGCA‐3′ | |
miR‐101 | F: 5′‐CTGTGCAGCAAACAGTCCAC‐3′ |
R: 5′‐AGAGAGGGTGCTACAGGAG‐3′ | |
RUNX1 | F: 5′‐CTCCGTGCTACCCACTCACT‐3′ |
R: 5′‐GTCGTTGAATCTCGCTACCTG‐3′ | |
RANTES | F: 5′‐CCCCATATGGCTCGGACACCA‐3′ |
R: 5′‐CTAGCTCATCTCCAATATGTTGAT‐3′ | |
CCR5 | F: 5′‐AACCTGGCCATCTCTGACCTG‐3′ |
R: 5′‐GTAGCAGATGACCATGAC‐3′ | |
TGF‐β1 | F: 5′‐TCGACATGGAGCTGGTGAAA‐3′ |
R: 5′‐GAGCCTTAGTTTGGACAGGATCTG‐3′ | |
Smad2 | R: 5′‐GTGTTTGCCGAGTGCCTAAGT‐3′ |
R: 5′‐TTACAGCCTGGTGGGATTTG‐3′ | |
U6 | F: 5′‐CTCGCTTCGGCAGCACA‐3′ |
R: 5′‐AACGCTTCACGAATTTGCGT‐3′ | |
β‐actin | F: 5′‐GGCATGGGTCAGAAGGATTCC‐3′ |
R: 5′‐ATGTCACGCACGATTTCCCGC‐3′ |
CCR5 indicates chemokine receptor 5; F, forward; miR, microRNA; R, reverse; RANTES, regulated upon activation normal T‐cell expressed and secreted; RT‐qPCR, reverse transcription quantitative polymerase chain reaction; RUNX1, runt‐related transcription factor 1; Smad2, Smad family member 2; TGF‐β1, transforming growth factor β1.