(A,B) Wholemount in situ hybridization detection of Armh4 mRNA in E12 embryo illustrating expression in the developing segmental dorsal root ganglia (DRGs) and in select cranial structures, with enlarged view of DRGs (arrows, B). (C,D) Wholemount Armh4 expression in E13.5 DRGs (arrows, C) and section in situ hybridization in transverse sections confirms Armh4 is present through the DRGs (arrows, D) and punctate expression is present within ventral regions of the neural tube (arrowheads, D). (E)
Armh4 is also expressed in the E12.5 frontonasal region, within the maxillary prominences (arrowheads) above the primordial mouth and either side (arrows) of the nasal septum. (F) E12.5 transverse view of head reveals Armh4 is expressed in the trigeminal ganglia (arrows), as well as telencephalon (tel) walls, median sulcus (arrowhead) and marginal layer around the forth (4th) ventricle. Scale bars: A,B =200μm; F=100μm. Abbreviations: e, eye; fl, forelimb; hl, hindlimb; mou, mouth; nt, neural tube.