Fig. 5.

Pretreatment of HUVECs with ANXA2 antibody reduced endothelial surface-associated S. aureus. Extracellular adhesive FITC-d-Lys-labeled bacteria in anti-ANXA2 antibody-treated HUVECs at 0.1, 1, and 3 μg/ml were detected by a SpectraMax i3× fluorescence plate reader at 15 min p.i. with S. aureus at 100 MOI. Normal mouse IgG was used as control IgG (normal IgG, 3 μ g/ml). All ECs were fixed with 4% paraformaldehyde as impermeable fixation. All experiments were repeated three times. There are 35 samples in each group. Data are represented as mean ± SEM. **Compared to the control group, P < 0.01