Table 2.
Detection of tumor-specific next-generation sequencing (NGS) mutations by ddPCR in samples obtained within the first month following surgery
| Sample origin | Sample type | NGS PTEN | NGS TP53 | ddPCR PTEN | ddPCR TP53 | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Allele frequency | Major allele (FW, RV) | Minor allele (FW, RV) | Allele frequency | Major allele (FW, RV) | Minor allele (FW, RV) | Allele frequency | Allele frequency | ||||||
| Right fallopian tube | tDNA | 76.3 | 173 | 276 | 585 | 959 | 75.0 | 498 | 597 | 1605 | 1908 | 78.0 | 75.0 |
| Colonic tumor | tDNA | 4.8 | 979 | 2052 | 54 | 103 | 4.2 | 3924 | 6031 | 181 | 274 | 3.3 | 5.1 |
| Sigmoid tumor | tDNA | 35.4 | 598 | 1010 | 350 | 549 | 35.8 | 1629 | 1885 | 917 | 1087 | 24.3 | 36.5 |
| Pelvic wash | cfDNA | 23.2 | 1184 | 1790 | 393 | 525 | 28.2 | 3553 | 4137 | 1443 | 1631 | 25.5 | 29.0 |
| Plasma day of surgery | cfDNA | 0.6 | 1201 | 2295 | 11 | 11 | 0.8 | 3963 | 4114 | 30 | 35 | 1.9 | 1.4 |
| Plasma 8 d postsurgery | cfDNA | neg. | n/a | n/a | n/a | n/a | 0.8 | 6744 | 7918 | 63 | 71 | 0.2 | 0.4 |
(NGS) Next-generation sequencing, (ddPCR) droplet digital polymerase chain reaction, (FW) forward, (RV) reverse, (tDNA) transfer DNA, (cfDNA) circulating free DNA.