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. 2016 Feb 1;24(4):205–216. doi: 10.1089/ars.2015.6377

FIG. 2.

FIG. 2.

SEC and spectroscopic analysis of E. granulosus IsTRP and Grx5. Size exclusion chromatography analysis of IsTRP and Grx5 is shown in (A, C), respectively, and was performed on a Superdex 75 10/300 GL column. Absorbance at 280, 320, and 420 nm is shown for EDTA-treated (ET) and nontreated (NT) samples. The UV-visible spectra and SDS-PAGE of fractions F1 (black) and F2 (gray) of the untreated samples are shown for IsTRP and Grx5 in (B, D), respectively. SDS-PAGE of recombinant proteins before (left lane) and after (right lane) gel filtration is shown as an inset. (E, F) Size exclusion chromatography of Fe/S reconstitution mixture for tag-free IsTRP and Grx5, respectively. Absorbance of the proteins AR and BR is shown. The spectra of the reconstituted proteins are shown in an inset. AR, after reconstitution; BR, before reconstitution; ET, EDTA-treated; NT, nontreated; SDS-PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.