FIG. 5.

Rescue of the ΔGrx5 mutant defects by Grx5 and IsTRP. (A) Growth plates after a 3-day incubation at 30°C on YPD. From left to right, 1:5 serial dilutions of exponential cultures were spotted. (B) Growth on YPD or YPG plates after 3 days at 30°C. S. cerevisiae Grx5 (ScGrx5), E. granulosus (EgGrx5), E. granulosus IsTRP (IsTRP), and null mutant (ΔGrx5) are shown. (C) Ratio between the Fe/S-containing enzyme aconitase and the non-Fe/S-containing enzyme malate dehydrogenase activities in exponential cultures at 30°C in YPGal medium. Values were normalized with respect to strain MML240 that expresses yeast Grx5-HA. The values are the mean of three experiments with standard deviations. Statistical analysis was performed using one-way ANOVA (*p < 0.05).