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Concentration Ratio of Iron and Sulfur to Holo-IsTRP
| [Fe]/[HoloProt] | [S2-]/[HoloProt] | |
|---|---|---|
| IsTRP | 0.9 ± 0.1 | 0.89 ± 0.07 |
Iron was measured using 4,7-diphenyl-1,10-phenanthroline, and acid-labile sulfur was measured using the methylene blue method (see the Materials and Methods section). Concentration of holoprotein in the analyzed fraction was estimated by analytical SEC.
IsTRP, iron–sulfur thioredoxin-related protein; SEC, size exclusion chromatography.
