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Concentration Ratio Between Low-Molecular-Weight Thiols and GSH and Apo or Holo Form of Grx5 and IsTRP Purified from Escherichia coli
| LMWT/Prot. | GSH/Prot. | |||
|---|---|---|---|---|
| Grx5 | IsTRP | Grx5 | IsTRP | |
| Holoprotein | 0.51 | 0.01 | 0.43 | <0.01 |
| Apoprotein | 0.11 | 0.01 | 0.09 | <0.01 |
GSH was added to all buffers during IMAC purification of both proteins, and free GSH was removed afterward by SEC.
GSH, glutathione; LMWT, low-molecular-weight thiols; IMAC, immobilized metal affinity chromatograph.
