Figure 5.

Effects of SHED on renal histopathological changes. (a) Representative images of PAS staining in the four groups. The PBS group displayed significant glomerular sclerosis, mesangial expansion, and tubular dilatation, while improvements in the glomeruli and tubules were observed in the SHED and BMSCs groups. (red arrow: glomerular sclerosis and mesangial expansion; blue arrow: tubular dilatation and protein cylinders; green arrow: renal tubular vacuolar degeneration). (b) IHC analysis of FN. FN increased significantly in the PBS group and remarkably decreased in the SHED and BMSCs groups. (c) Electron microscopy. The GEM was obviously thickened, and the foot processes of the podocytes were condensed and missing or in disarray in the PBS group. The thickness of the GEM and the foot processes of the podocytes improved in the SHED group and BMSCs group. Bar = 100 μm.