Table 1.

Primers used to amplify rickettsial genes. These primers were used to amplify outer membrane protein A (ompA), outer membrane protein B (ompB), gene D (sca4), citrate synthase (gltA), and 16S rRNA.

Gene	Fragment Size	Primers	Reference
ompA	540-bp	190-FN1 5′-AAGCAATACAACAAGGTC 190-RN1 5′-TGACAGTTATTATACCTC	(Paddock et al., 2004)
ompB	1895-bp	RompB11F 5’-ACCATAGTAGCMAGTTTTGCAG Rompl902R 5’CCGTCATTTCCAATAACTAACTC	(Jiang et al., 2005)
sca4	928-bp	D1F 5’-ATGAGTAAAGACGGTAACCT D928R 5’- AAGCTATTGCGTCATCTCCG	(Jiang et al., 2005)
gltA	830-bp	CS239 5′ GCTCTTCTCATCCTATGGCTATTAT CS1069 5′ CAGGGTCTTCGTGCATTTCTT	(Labruna et al., 2004)
16S rRNA	757-bp	Ric 5’- TCTAGAACGAACGCTATCGGTAT Ric Rt 5’- TTTCATCGTTTAACGGCGTGGACT	(Nilsson et al., 1997)
16S rRNA	1385-bp	Ric 5’- TCTAGAACGAACGCTATCGGT AT Ric U8 5’- TGCGTTAGCTCACCACCTTCAGG	(Nilsson et al., 1997)