Skip to main content
. Author manuscript; available in PMC: 2019 Dec 16.
Published in final edited form as: Sci Immunol. 2019 May 17;4(35):eaax0644. doi: 10.1126/sciimmunol.aax0644

Figure 4. Simultaneous emAb expression from both heavy chain loci.

Figure 4.

(A) Representation of a productively recombined heavy chain and a non-productive heavy chain, both of which contain the huIgH296 gRNA target site. (B) Representative flow cytometric analysis of the loss of Igλ expression by FACS-purified Igλ+ primary cells engineered to express Flu-emAb, which utilizes Igκ. The numbers on the Igλ plots represent the mean ± SD% from two independent experiments. (C) Representative flow cytometric analysis of RSV F antigen and Flu HA binding by control B cells, B cells engineered separately with Flu-emAb and RSV-emAb and co-cultured for 24 hours prior to analysis, or B cells simultaneously double-engineered with Flu-emAb and RSV-emAb. Data are representative of two independent experiments.