Figure 5.

AtRTP5 transcription was induced by the infection of Phytophthora parasitica and nuclear localization of AtRTP5 protein was required for function in plant immunity. (a) Recombinant protein GFP‐AtRTP5 was localized in the nucleus and plasma membrane. The fluorescence of GFP‐AtRTP5 overlapped with that of Myr‐mCherry (upper panel). The blue fluorescence of the DAPI‐stained nucleus could merge with green fluorescence (lower panel). (b) The protein was extracted from Nicotiana benthamiana leaves expressing GFP‐AtRTP5 and a western blot showed the correct protein band of GFP‐AtRTP5 (c. 106 kDa). (c) Myr peptide was added to the N terminus of AtRTP5‐GFP, which resulted in the fusion protein transferring to the plasma membrane from the nucleus. Myr‐AtRTP5‐GFP had no function of promoting pathogen infection. Ten leaves were tested in each experiment and three independent experiments were performed. (d) The expression pattern of AtRTP5 determined by qRT‐PCR indicated that AtRTP5 was up‐regulated during the infection of P. parasitica. Total RNA was obtained from detached leaves infected by P. parasitica at different times. All scale bars in (a) and (c) are 40 μm.