Figure 3.

The expression of CTB1 and CTB8 relative to β‐tubulin in Cercospora cf. flagellaris wild‐type (WT) and ∆avr4 mutants. Fungal RNA was isolated from cultures grown in potato dextrose broth under light or darkness for 5 days. The expressions of CTB1 and CTB8 genes were normalized to that of β‐tubulin from the same sample. Data are the mean and standard errors of three different experiments with three biological replicates of each fungal isolate and three technical replicates of real‐time reverse transcription PCRs. Asterisks (*) indicate significant difference between the wild‐type and the mutants when grown under light or dark conditions (P < 0.05).