Figure 6.

Virulence of Cercospora cf. flagellaris wild‐type (WT) and ∆avr4 mutants on detached soybean leaves. (A) Detached soybean leaves were inoculated with mycelia plugs of the WT or ∆avr4 mutants and disease severity was determined at 14 days after inoculation. (B) Disease severity was determined based on the 1–5 rating scale (1 = less and 5 = more severe). Data are the mean and standard errors of three different experiments with three biological replicates (eight leaves) within each fungal isolate. Asterisks (**) indicate significant disease severity difference between the wild‐type and the mutants (P < 0.01). (C) Fungal growth was quantitatively assessed by determining the fungal DNA levels in soybean leaves through real‐time PCR. Data are the mean and standard errors of three different experiments with three biological replicates (eight leaves) for each wild‐type or mutant.