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. 2019 Oct 23;21(1):53–65. doi: 10.1111/mpp.12879

Figure 7.

Figure 7

The effect of Avr4 dsRNA on cercosporin production and target gene expression. (A) Visual phenotype changes of the wild‐type (WT) Cercospora cf. flagellaris grown in half‐strength complete medium (CM) alone or treated with total nucleic acid extracted from Escherichia coli containing L4440 vector (WT + EV dsRNA) or containing Avr4 gene‐specific dsRNA (WT + Avr4 dsRNA) for 4 days in comparison to that of ∆avr4 mutant. The final concentration in the culture was 16 µg/mL for either EV dsRNA or Avr4 dsRNA. (B) Bar graph of cercosporin production in non‐treated WT C. cf. flagellaris grown in half‐strength CM or treated with total nucleic acid extract containing L4440 vector (EV) or containing Avr4 gene‐specific dsRNA (Avr4 dsRNA). (C) Visual comparison of cercosporin extracted from the WT C. cf. flagellaris under different treatments for 4 days. (D) Relative expressions of Avr4, CTB1 and CTB8 genes in the WT C. cf. flagellaris after different treatments.