Table 1.
Primers and probes used in this study.
| System name | Primer & probe name | Sequences 5′–3′ | Specificity | References |
|---|---|---|---|---|
| Pan-fil 28S qPCR-based system | qFil-28S-F | TTGTTTGAGATTGCAGCCCA | Filariae | [22] |
| qFil-28S-P | 6FAM-5′-CAAGTACCGTGAGGGAAAGT-3′-TAMRA | |||
| qFil-28S-R | GTTTCCATCTCAGCGGTTTC | |||
| All-Wol 16S qPCR-based system | all.Wol.16S.301-F | TGGAACTGAGATACGGTCCAG | Wolbachieae | |
| all.Wol.16S.347-P | 6FAM-5′-AATATTGGACAATGGGCGAA-3′-TAMRA | |||
| all.Wol.16S.478-R | GCACGGAGTTAGCCAGGACT | |||
| Triplex TaqMan COI qPCR-based system | Fil.COI.749-F | CATCCTGAGGTTTATGTTATTATTTT | ||
| D.imm.COI.777-P | 6FAM-CGGTGTTTGGGATTGTTAGTG-TAMRA | Dirofilaria immitis | ||
| D.rep.COI.871-P | 6VIC-TGCTGTTTTAGGTACTTCTGTTTGAG-TAMRA | Dirofilaria repens | ||
| A.rec.COI.866-P | Cy5-TGAATTGCTGTACTGGGAACT-BHQ-3 | Acanthocheilonema reconditum | ||
| Fil.COI.914-R | CWGTATACATATGATGRCCYCA | |||
| Duplex Wol-Diro ftsZ qPCR-based system | WDiro.ftsZ.490-F | AAGCCATTTRGCTTYGAAGGTG | Wolbachia endosymbiont of D. immitis and D. repens | |
| WDimm.ftsZ.523-P | 6FAM-CGTATTGCAGAGCTCGGATTA-TAMRA | |||
| WDrep.ftsZ.525-P | 6VIC-CATTGCAGAACTGGGACTGG-TAMRA | |||
| WDiro.ftsZ.600-R | AAACAAGTTTTGRTTTGGAATAACAAT | |||
| Duplex HWs COI qPCR-based system | Hw.COI.723-F | TCAGCATTTGTTTTGGTTTTT | ||
| D.imm.COI.777-P | 6FAM-CGGTGTTTGGGATTGTTAGTG-TAMRA | D. immitis | ||
| A.vas.COI.813-P | 6VIC-TGACTGGGAAGAAGGAGGTG-TAMRA | Angiostrongylus vasorum | ||
| Hw.COI.950-R | GCASTAAAATAAGYACGAGWATC | |||
| Pan-Nematoda primers 18S PCR-based system | Fwd.18S.631 | TCGTCATTGCTGCGGTTAAA | Nematoda | This study |
| Rwd.18S.1825r | GGTTCAAGCCACTGCGATTAA |