Table 2.
Screening for dirofilariosis in a military kennel in the Indre department (central France).
| Dog number (No.) | Breed* | Age (year) | Kennel presence time (year) | Parasitological diagnosis: Knott test | Serological screening |
Molecular detection of filarial DNA using the qPCR Pan-Filaria 28S | Genotyping: 18S rRNA gene | Molecular identification of filarial species using a COI Triplex qPCR-based system |
Molecular identification of Wolbachia genotypes using ftsZ duplex qPCR-based system |
Diagnosis | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Witness® Dirofilaria | DiroCHEK® | Dirofilaria immitis | Dirofilaria repens | Acanthocheilonema reconditum | Wolbachia endosymbiont of D. immitis | Wolbachia endosymbiont of D. repens | ||||||||
| 1 | BSM | 10 | 8.5 | Neg. | Neg. | Neg. | Neg. | NE** | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 2 | BSM | 7 | 6 | D. repens | Pos. | Pos. | Pos. | D. repens | Neg. | Pos. | Neg. | Pos. | Pos. | Occult heartworm and subcutaneous dirofilariosis |
| 3 | GS | 6 | 5 | D. immitis | Pos. | Pos. | Pos. | D. immitis | Pos. | Neg. | Neg. | Pos. | Neg. | Heartworm disease |
| 4 | BSM | 6 | 5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 5 | BSM | 6 | 5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 6 | BSM | 6.5 | 5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 7 | GS | 6 | 4 | D. immitis + D. repens | Neg. | Pos. | Pos. | NE | Pos. | Pos. | Neg. | Pos. | Pos. | Heartworm and subcutaneous dirofilariosis |
| 8 | BSM | 4.5 | 3 | Neg. | Neg. | Neg. | Pos. | NE | Pos. | Pos. | Neg. | Pos. | Pos. | Occult heartworm and subcutaneous dirofilariosis |
| 9 | GS | 3.5 | 2.5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 10 | BSM | 4 | 2 | D. repens | Neg. | Neg. | Pos. | D. repens | Neg. | Pos. | Neg. | Pos. | Pos. | Occult heartworm and subcutaneous dirofilariosis |
| 11 | BSM | 3 | 2 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 12 | GS | 3 | 2 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 13 | GS | 4 | 1.5 | D. repens | Pos. | Pos. | Pos. | D. repens | Neg. | Pos. | Neg. | Pos. | Pos. | Occult heartworm and subcutaneous dirofilariosis |
| 14 | GS | 2.5 | 1.5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 15 | BSM | 3.5 | 1 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 16 | BSM | 2 | 0.5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
| 17 | BSM | 2 | 0.5 | Neg. | Neg. | Neg. | Neg. | NE | Neg. | Neg. | Neg. | Neg. | Neg. | Healthy dog |
*
BSM: Belgian shepherd malinois, GS: German shepherd.
**
NE: Not evaluated.