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. 2019 Dec 13;7:e8143. doi: 10.7717/peerj.8143

Figure 5. The effect of embryo aggregation on transcript levels of stress-related genes.

Figure 5

Real-time PCR results for (A) ER-stress related genes (ATF4, CHOP, IRE1), (B) ROS related genes (SOD1, SOD2, Catalase) and (C) mitochondria related genes (TFAM, POLG, POLG2) at blastocysts in the indicated groups. All genes were normalized with the GAPDH gene. (*P < 0.05). For all panels, n indicates number of embryos examined. Re = 3. 1X; one zona-free embryo, 3X; three zona-free embryos.