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. Author manuscript; available in PMC: 2020 Jun 2.
Published in final edited form as: Nat Cell Biol. 2019 Dec 2;21(12):1544–1552. doi: 10.1038/s41556-019-0427-x

Fig. 3. Live-imaging of differentiating Deup1−/− ependymal cells reveals normal, step-wise kinetics of centriole amplification.

Fig. 3.

(a) Box (25 to 75%) and whisker (10 to 90%) plots of A- (Gray), G- (Orange), and D- (Green) phase duration in differentiating Centrin 2-GFP Deup1+/+ and Deup1−/− ependymal cells using time-lapse microscopy. A = amplification phase, G = growth phase, D = disengagement phase; n > 3 cultures/genotype. The total number of cells per genotype at each phase (# cells) is indicated. P values, unpaired, two-tailed, Welch’s t-test. n.s.=not statistically significant (p > 0.05).

(b) Still images from time-lapse videos of Centrin 2-GFP Deup1+/+ (Supplementary Video 1) or Deup1−/− (Supplementary Video 2) ependymal cells in the amplification (A), growth (G) or disengagement (D) phase. Arrow heads and zoomed regions mark the parent centrioles. X marks Centrin 2-GFP aggregates resulting from expression of the Centrin 2-GFP transgene25. Scale bars represent 2 μm.