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. 2019 Dec 2;19(1):443–450. doi: 10.3892/etm.2019.8263

Figure 3.

Figure 3.

Effect of miR-221 inhibitor on HUVECs. (A) HUVECs were transfected with inhibitor control or miR-221 inhibitor for 48 h then, the level of miR-221 in HUVECs was detected using reverse transcription-quantitative PCR. (B) Cell viability was measured by an MTT assay. (C) Cell migration and (D) invasion were determined using Transwell and Matrigel assays, respectively. (E) Representative flow cytometry plots and (F) quantification of cell apoptosis was measured using flow cytometry. (G) Protein level of Bax and Bcl-2 was detected by western blot analysis. (H) Tube formation assay was used to determine cell tube formation ability. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. inhibitor control. miR, microRNA; HUVECs, human umbilical vein endothelial cells; PI, propidium iodide; FITC, fluorescein isothiocyanate.