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. 2019 Dec 2;19(1):443–450. doi: 10.3892/etm.2019.8263

Figure 5.

Figure 5.

Effect of miR-221 mimic on the PTEN/PI3K/AKT pathway in HUVECs. (A) Representative blots of HUVECs following transfection with mimic control, miR-221 mimic, miR-221 mimic + control-plasmid or miR-221 mimic + PTEN-plasmid. (B) Quantification of p-AKT protein level was measured by western blotting. (C) PTEN mRNA levels in HUVECs detected using reverse transcription-quantitative PCR. Data were presented as the mean ± standard deviation. **P<0.01 vs. Mimic control; ##P<0.01 vs. Mimic + control-plasmid. miR, microRNA; HUVECs, human umbilical vein endothelial cells; PTEN, phosphatase and tensin homolog; p, phosphorylated.