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. 2019 Dec 1;2019:1871394. doi: 10.1155/2019/1871394

Figure 3.

Figure 3

Exosome from ARDS patients' plasma delivered less miR-425 into target cells and did not repress PTEN expression. (a) A549 and HFL-1 cells were cocultured with exosomes from ARDS patients or healthy controls. The miR-425 level was detected by qRT-PCR 48 hours after coculture. (b) A549 and HFL-1 cells were transfected with PTEN reporter and then cocultured with exosomes from ARDS patients or healthy controls. The luciferase activity was detected 48 hours after coculture. Results were analyzed by t-test, and p < 0.05 was considered significant. ∗p < 0.05, ∗∗P < 0.01. (c) A549 and HFL-1 cells were cocultured with exosomes from ARDS patients or healthy controls. The PTEN protein level was determined by immunoblotting.