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. 2019 Nov 21;10(12):6408–6421. doi: 10.1364/BOE.10.006408

Fig. 5.

Fig. 5.

Analysis of NAD(P)H lifetime dynamics in apoptosis-resistant cell line (BAX/BAK DKO) compared to the apoptosis non-resistant cell line (WT). (a-h) Snapshots of (a-d) WT and (e-h) BAX/BAK DKO cells at (a, e) 0 seconds, (b, f) 20 seconds, (c, g) 120 seconds, and (d, h) 15 minutes following 1 µM STS treatment (see Visualization 2 (4.3MB, mp4) ). (i) Short-term tracking of NAD(P)H lifetime dynamics reveals a statistically significant increase in the NAD(P)H lifetime of WT cells compared to DKO cells at approximately 120 seconds following treatment (p < 0.01). (j) NAD(P)H lifetime at later times (up to 48 hours) shows similar NAD(P)H lifetime values for WT and DKO cells. Scalebar is 50 µm.