Fig. 6.

LSLFM imaging of the zebrafish brain expressing GCaMP6s in vivo produced more detail than WFLFM imaging. Images of the zebrafish brain acquired with (a) WFLFM, (b) LSLFM, (c) direct light-field imaging, and (d) LSM at depths of −16 µm, 0 µm, and 16 µm. LSLFM revealed the structure of sub-regions within the larval zebrafish brain, comparable to direct light-field imaging. WFLFM failed to resolve such structure due to the high background. Right: We quantified the feature size as the distance between the half feature heights. Scale bar: 50 µm.