Fig. 3.

DUET dual-mode imaging. An H&E slide is illuminated from below using a high color rendering index white LED and a color image is acquired; the white LEDs are turned off, and a 405-nm LED then is used in standard epifluorescence mode to excite fluorescence signals that are then collected using the same light path and camera. The two image modes are naturally pixel-registered. The process is repeated at each position of a whole-slide scanning procedure, using an XY stage and 10X lens. Brightfield image of human (B) kidney (C) liver and (D) breast from H&E slide. E-F: fluorescence images of human kidney, liver and breast from the same regions shown on B, C and D.