Fig. 5.

RGB images of H&E-stained slide fluorescence. Top row: kidney; middle row: ovarian cancer; bottom row: breast cancer. Phasor plots are shown in the middle column, with unmixed collagen signals shown as monochrome images and as blue overlays on top of the original brightfield images. Compared to the multispectral phasor plot shown in Fig. 4, these plots have fewer clusters, and it is not possible to separate collagen from basement membrane signals. The shapes of the phasor clusters differ from each other, reflecting the fact the slides were stained in different laboratories, and that 3 different color cameras (CCD, CMOS and 3-band filter-wheel-equipped monochrome) were used to capture these images.