Fig. 4.

Normal free-running circadian periods and hypoactivity in Shank3b^−/−, Cntnap2^−/−, Pcdh10^+/−, and Fmr1 KO mice during constant darkness. A-D) Relative to sex-matched WT controls, there are no differences in free-running circadian period (tau) in (A) Shank3b^−/− males (23.60 ± 0.02 h, WT: 23.64 ± 0.02 h; p = 0.25) or females (23.62 ± 0.03 h, WT: 23.67 ± 0.02 h; p = 0.25), (B) Cntnap2^−/− males (23.61 ± 0.02 h, WT: 23.61 ± 0.02 h; p =0.93) or females (23.65 ± 0.04 h, WT: 23.64 ± 0.03 h; p= 0.64), (C) Pcdh10^+/− males (23.53 ± 0.04 h, WT: 23.54 ± 0.08 h; p= 0.87) or females (23.51 ± 0.05 h, WT: 23.50 ± 0.03 h; p =0.83), or (D) Fmr1 KO males (23.67 ± 0.02 h, WT: 23.64 ± 0.02 h; p =0.26). (E–L) Peak activity patterns during DD (1-h bins) are similar to activity patterns observed during 12h:12 h LD. (E,I) Shank3b^−/− male and female mice have lower peak activity in DD than sex-matched WT controls. (F,J) Relative to sex-matched WT in DD, male Cntnap2^−/− mice are not hypoactive in the (F) horizontal axis, but are hypoactive in the (J) vertical direction. Female Cntnap2^−/− mice are not hypoactive relative to sex-matched WT in DD. (G,K) Pcdh10^+/− males but not females have lower peak activity levels in DD compared to WT. (H,L) Fmr1 KO males do not have altered peak activity levels in the (H) XY-axis, but display reduced peak activity in the (L) Z-axis during DD in comparison to WT littermates. Mean ± standard error of the mean (s.e.m.) *p < 0.05, **p < 0.01, ***p < 0.001.