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. 2019 Dec 9;9(1):1698889. doi: 10.1080/20013078.2019.1698889

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Complement receptor activation induces enrichment of granule proteins in EVs. a. Label-free quantitative analysis of granule protein presence in different EVs derived from human PMN. Analysis of one out of 2 similar experiments. b. Label-free quantitative analysis of proteomics data on typical granule proteins of sEV and aEV obtained from untreated or dasatinib pretreated human PMN. Dotted line represents sEV data as value 1; mean and individual values normalized to sEV; line represents mean ± SD. c. Representative Western blot of lactoferrin (LTF), myeloperoxidase (MPO) and actin in EVs. Representative images out of 11 or 6 independent experiments. d. Densitometric analysis of the Western blots. LTF and MPO were related to actin. Data were compared by using two-way ANOVA coupled with Tukey’s post hoc test; n = 11, 6. Level of significance is indicated by * for LTF and by # for MPO.