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. 2019 Dec 9;9(1):1698889. doi: 10.1080/20013078.2019.1698889

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Characterization of murine PMN derived EVs. a. Quantification of EVs produced spontaneously (sEV) and after activation with non-opsonized (zEV) or completely (CabzEV) opsonized zymosan. Data were compared to sEV using one-way ANOVA coupled with Dunnett’s post hoc test; n = 32. Representative electron microscopic images of sEV (b.) and CabzEV (c.) prepared from WT murine PMN. Original magnification is 30,000×. Representative pictures out of 3 similar experiments. d. Size distribution spectra of murine EVs based on electron microscopy images, data originate from 200 EVs pro samples. The dotted line represents the possible limit of detectability by flow cytometry. e. Size distribution spectra of EVs measured by DLS. Broken line represents 0.1% Triton X-100 treated aEV. Signal above 1000 nm is due to zymosan contamination. Representative results out of 3 independent experiments.