Table 3.

Effect of species, CO₂ concentration (390, 520 ppm), climatic condition (Clim) (Con, ECE), date (D1, D2), and their interactions (based on ANOVA) on water content, cell membrane stability (CMS), soluble carbohydrate content, total fructans:sucrose ratio, and low DP:sucrose ratio in leaf meristems of D. glomerata and H. lanatus

Variable		Species	CO2	Clim	Species× CO2	Species× Clim	CO2× Clim	Species× CO2xClim	Date	Date× Species	Date× CO2	Date× Clim
	Water content	0.004	NS	<0.001	NS	NS	0.050	NS	<0.001	NS	NS	<0.001
	CMS	NS	NS	<0.001	NS	0.009	NS	NS	0.003	NS	NS	<0.001
Soluble carbohydrate content (mg g DM−1)	Fructans	<0.001	0.042	NS	NS	NS	NS	NS	<0.001	NS	NS	NS
	Sucrose	<0.001	NS	<0.001	NS	NS	0.038	NS	NS	NS	NS	<0.001
	Glucose	0.027	NS	NS	NS	<0.001	NS	NS	0.004	NS	NS	NS
	Fructose	NS	NS	NS	NS	0.004	NS	NS	<0.001	NS	NS	NS
Fructan composition (relative content, %)	Low DP	<0.001	NS	NS	NS	0.004	NS	NS	NS	NS	0.018	NS
	Medium DP	NR	NS	NS	NR	NR	NS	NR	<0.001	NR	NS	NS
	High DP	NR	NS	NS	NR	NR	NS	NR	0.012	NR	NS	NS
Fructans:sucrose ratios	Total fructans:sucrose	<0.001	NS	<0.001	NS	NS	NS	NS	NS	NS	NS	<0.001
	Low DP:sucrose	0.001	NS	0.002	–	–	–	–	NS	–	–	–
	Medium DP:sucrose	NR	NS	<0.001	NR	NR	NS	NR	NS	NR	NS	<0.001
	High DP:sucrose	NR	NS	NS	NR	NR	NS	NR	NS	NR	NS	0.004
Fructans:fructans ratios	Low DP:Medium DP	NR	NS	NS	NR	NR	–	NR	NS	NR	–	–
	Low DP:High DP	NR	NS	NS	NR	NR	NS	NR	0.0037	NR	0.019	NS
	Medium DP:High DP	NR	NS	NS	NR	NR	–	NR	NS	NR	–	–

For medium and high DP composition, medium DP:sucrose, high DP:sucrose and fructans:fructans ratios, the effect of species was not relevant (NR) since groups of medium and high DP were not similar in the two species. For the ratios of low DP:sucrose; low DP:medium DP, and medium DP:high DP, the effects of CO₂, Clim, and date were analysed independently by non-parametric Kruskal–Wallis tests. P values are shown when <0.05. NS, Non-significant effect. –, Conditions for analysis not met.