Fig. 1.

Detection of HvCslF6 protein and YFP fluorescence in developing barley grain. (A) Total HvCslF6 protein levels in the transgenic grains are slightly higher in ‘outer tissues' and clearly higher in endosperm tissues relatively to the WT at 15 DAP. The arrow indicates HvCslF6 protein at 89–100 kDa. (B–D) Fluorescence from YFP is detected in the peripheral endosperm of barley grain at 20 DAP. Images (B) and (C) are under ×1 magnification with scale bars equivalent to 1000 µm. Image (D) is under ×10 magnification with a scale bar equivalent to 100 µm. Sections for image (C) were prepared in the equatorial part of the grain. *Endosperm sample includes embryo tissues. Abbreviations: aleurone (al), central endosperm (ce), embryo (em), pericarp (p), peripheral endosperm (pe).