FIG. 3.

Supernatants from MSC:DC cocultures prevent full maturation of LPS-stimulated DC. DC were cocultured with MSC (1:10 ratio, MSC:DC) that were either untreated or treated with IFNγ. In parallel studies, DC were cultured with supernatants obtained from MSC:DC cocultures. The DC were then activated with LPS (50 ng/mL) for 24 hr. The DC were subsequently analyzed for expression of MHCII (A), CD86 (B), and (C) CD40 by flow cytometry. Data are representative of three independent experiments. P values calculated for statistical variance were determined using the Mann–Whitney test. (“ns”, not significant; *P < 0.05, **P < 0.01, and ***P < 0.005).