FIG. 6.

Effect of MSC on DC maturation and antigen endocytosis. Cultures of 1 × 10⁴ DC in 24-well plates were cultured with or without 1 × 10³ IFNγ-treated MSC and pulsed with APC-conjugated OVA (final concentration 5 μg/mL) at time = 0, and then DC were harvested and processed for flow cytometry at the indicated time points. As a control, MSC alone were pulsed and harvested identically. Vertical lines represent the range of APC MFI for each time point in triplicate experiments. Result is representative of three DC sets from three separate dogs. P values for statistical variance at the 24 hr time point were determined using Repeated Measures ANOVA with Tukey's multiple comparison tests. *P < 0.05, **P < 0.01, and ***P < 0.005.